Disruption of the Expression of the Cinnamoyl–CoA Reductase (CCR) Gene OsCCR18 Causes Male Sterility in Rice (Oryza sativa L. japonica)

The biological process of anther development is very complex. It remains largely unclear how the cinnamoyl–CoA reductase (CCR) encoding genes function in regulation plants. Here, we establish that CCR family gene OsCCR18 essential for maintaining male fertility rice. transcripts were greatly abundant panicles at S4 and S5 developmental stages subcellular localization proteins was nucleus knockout resulted a severely abnormal degradation tapetum as well granular Ubisch bodies, leading to inability form normal pollen mutants. Compared with wild–type (WT) rice, osccr18 mutants had no visible grains entirely sterility. Furthermore, several development–related genes, including OsPDA1, OsDTD, OsC6, OsACOS12, OsTDR, OsWDA1, OsDPW, OsCYP703A3, OsNOP, significantly lower expressed from S8 than WT Additionally, hundreds involved phenylpropanoid biosynthesis, fatty acid synthesis metabolism exhibited distinct expression patterns between mutants, which may be crucial controlling These findings add new regulatory role gene–mediated